![]() Campbell, Shawn O.įarrel, Biochemistry sixth edition (2009) Thomson Higher Education Corporation. Bowen, Understanding Biotechnology, (2003), Pearson Education, Inc. Yamada, Short Protocols in Cell Biology: A Compendium of Methods from Current Protocols in Cell Biology, (2004), John Wiley and Sons, Inc., New Jersey. Harford, Jennifer Lippincott-Schwartz and Kenneth M. Colin Ratledge, Bjorn Kristiansen, Basic Biotechnology, Second Edition, (2001) Cambridge University Press. Other than that, medicine administration require suitable dose for each patient with variable needs. Then, count the dilution factor and times it with the actual volume of the sample. Repeat the steps until the cells can be observed under the microscope when the diluted sample was observed. Serial dilution is a simple yet efficient technique to determine the number of cells or organisms in a concentrated sample.įirst, take a portion of the sample and does serial dilution on it. This method is applicable in several fields, not only in chemistry. By knowing the dilution factor of certain solution, the calculations of the concentration become easier and systematic. Other than that, the diluted solution from this serial dilution can be used to count the concentration of the actual solution. This is to ensure that the exact concentration can be obtained for the experiment to become success. Serial dilution comes in handy when the solution is too concentrated to be used in experiments or ingredients preparation. There are several benefits of performing serial dilution. In serial dilution, the entire dilution factor will be multiple together to be the actual dilution factor. The factor can be obtained by dividing the actual volume of the initial chemical used to the final volume of the chemical after water is added.Īs example, if 1.0 ml of 3M HCl is added with 9 ml of distilled water, the dilution factor becomes 1/10. One way is by obtaining a factor called the Dilution Factor (DF). After performing the dilution, we need to know how much difference are the diluted chemical and the initial, undiluted ones. Thus, serial dilution simply means a series of repeated dilution performed on the same chemical basically to change its concentration. ![]()
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